Over the past year, increased regulatory pressure in multiple regions like UK OFCOM and Australia's eSafety has led to higher operational costs, including infrastructure, security, and the need to work with more specialized service providers to keep the site online and stable.
If you value the community and would like to help support its continued operation, donations are greatly appreciated. If you wish to donate via Bank Transfer or other options, please open a ticket.
Donate via cryptocurrency:
Bitcoin (BTC):
Ethereum (ETH):
Monero (XMR):
Broke metal seal on bottle of N
Thread startervioletdevil
Start date
You are using an out of date browser. It may not display this or other websites correctly. You should upgrade or use an alternative browser.
I broke all of the metal seal, took the blue cap off and everything. All that's left is the rubber stopper. Does this expose it to oxygen? If so, how long do I have before I need to take it? and do I need to refrigerate it?
Reactions:
som1, PeacefulTonic, dyingalone123 and 1 other person
When the metal part is off, it's no longer sterile and being exposed to the air. It will last for about a month after that and putting it in a fridge is recommended. This is the information that I'm aware of, if someone wants to correct me they're welcome to do so.
Reactions:
shrek34, som1, dyingalone123 and 1 other person
When the metal part is off, it's no longer sterile and being exposed to the air. It will last for about a month after that and putting it in a fridge is recommended. This is the information that I'm aware of, if someone wants to correct me they're welcome to do so.
To do a sleep test. But I could've just opened the middle seal up. I didn't realize that opening the metal seal would expose it to air. Thought just the rubber stopper needed to be on.
I broke all of the metal seal, took the blue cap off and everything. All that's left is the rubber stopper. Does this expose it to oxygen? If so, how long do I have before I need to take it? and do I need to refrigerate it?
I broke all of the metal seal, took the blue cap off and everything. All that's left is the rubber stopper. Does this expose it to oxygen? If so, how long do I have before I need to take it? and do I need to refrigerate it?
If you haven't removed the rubber stopper you're fine; that element is airtight compressed in the bottle's neck. If you can't keep it in the fridge, keep it in a dark cool place. Watch for any significant discoloration and precipitate at the bottom of the bottle.
You can try this little experiment: turn the bottle up side down and see if it leaks (it shouldn't), after holding it in that position, see if you se small bubbles of air going up from the neck to the bottom of the bottle (you shouldn't see any).
Reactions:
siray, LastLoveLetter, PeacefulTonic and 3 others
If you haven't removed the rubber stopper you're fine; that element is airtight compressed in the bottle's neck. If you can't keep it in the fridge, keep it in a dark cool place. Watch for any significant discoloration and precipitate at the bottom of the bottle.
You can try this little experiment: turn the bottle up side down and see if it leaks (it shouldn't), after holding it in that position, see if you se small bubbles of air going up from the neck to the bottom of the bottle (you shouldn't see any).
That's interesting to learn. Although from some pictures I've encountered, the bottles D sends seem to be manufactured/designed somewhat differently than the ones A used to provide. I could be wrong though.
That's interesting to learn. Although from some pictures I've encountered, the bottles D sends seem to be manufactured/designed somewhat differently than the ones A used to provide. I could be wrong though.
I was going to put my N bottles in an airtight container. Any thoughts on this idea? I was also going to put little silica gel pouches next to the bottles.
I was going to put my N bottles in an airtight container. Any thoughts on this idea? I was also going to put little silica gel pouches next to the bottles.
It won't hurt to put them in an airtight container as it's a further barrier to extreme or quick temperature changes but it's not necessary if they're stores in a dark stably cool place. The silica gell is definitely overkill but again, I don't see it hurting anything. This is all assuming your bottles are still unopened and have their seals intact.
A good place to store them in their original packaging might be in a shoe box in a cellar or similar. Some use the fridge. In those cases it's important to avoid freezing apparently.
If taking samples out using a syringe you should void the syringe of air and tip the bottles upside down to extract from them. Doing so correctly will stop air entering the empty space within the bottle so shouldn't impact it's shelf life. It's seal is self sealing eubber so will not leak either. It's should be obvious but I'll say it.... use low guage/small needled syringes in order to keep the puncture hoke as small as possible and less chance of air to penetrate in between punctures/healing.
It won't hurt to put them in an airtight container as it's a further barrier to extreme or quick temperature changes but it's not necessary if they're stores in a dark stably cool place. The silica gell is definitely overkill but again, I don't see it hurting anything. This is all assuming your bottles are still unopened and have their seals intact.
A good place to store them in their original packaging might be in a shoe box in a cellar or similar. Some use the fridge. In those cases it's important to avoid freezing apparently.
If taking samples out using a syringe you should void the syringe of air and tip the bottles upside down to extract from them. Doing so correctly will stop air entering the empty space within the bottle so shouldn't impact it's shelf life. It's seal is self sealing eubber so will not leak either. It's should be obvious but I'll say it.... use low guage/small needled syringes in order to keep the puncture hoke as small as possible and less chance of air to penetrate in between punctures/healing.
Thanks for the input, appreciate it. Yeah, I won't be opening the bottles until I drink them. I trust D and the people on here tested it and said it was good. I won't be putting them in a fridge either. I'll just put them in a nice cool cupboard inside an airtight container with those little silica gel thingies. Overkill is my thang hahahah
It won't hurt to put them in an airtight container as it's a further barrier to extreme or quick temperature changes but it's not necessary if they're stores in a dark stably cool place. The silica gell is definitely overkill but again, I don't see it hurting anything. This is all assuming your bottles are still unopened and have their seals intact.
A good place to store them in their original packaging might be in a shoe box in a cellar or similar. Some use the fridge. In those cases it's important to avoid freezing apparently.
If taking samples out using a syringe you should void the syringe of air and tip the bottles upside down to extract from them. Doing so correctly will stop air entering the empty space within the bottle so shouldn't impact it's shelf life. It's seal is self sealing eubber so will not leak either. It's should be obvious but I'll say it.... use low guage/small needled syringes in order to keep the puncture hoke as small as possible and less chance of air to penetrate in between punctures/healing.
Yeah put the syringe in and extract a little. The stopper on top is self healing so it won't leave a hole that leaks. Make sure there is no air in the syringe before putting it in. The aim is to avoid any air getting in as it aids degredation of the N but as long as your syringe plunger is fully down there will be no air in it and its a very simple case of tipping the bottle up and pulling the plunger to fill the syringe.
Yeah put the syringe in and extract a little. The stopper on top is self healing so it won't leave a hole that leaks. Make sure there is no air in the syringe before putting it in. The aim is to avoid any air getting in as it aids degredation of the N but as long as your syringe plunger is fully down there will be no air in it and its a very simple case of tipping the bottle up and pulling the plunger to fill the syringe.
this vid is saying to put air in the bottle… so what's the worst that can happen if you don't put air into it? Do you like, pressurize it or something?
this vid is saying to put air in the bottle… so what's the worst that can happen if you don't put air into it? Do you like, pressurize it or something?
This is not correct procedure for testing. Do not inject air into the vial. I give myself injections for a medical condition and first thing I do is force the air out of the syringe. What she is demonstrating is not what you would be doing if you decide to test, which frankly I would recommend against.
You have told me about OCD or something that is causing you to continually question what you are being told. We are telling you that forcing air into your bottle will facilitate degradation of its effectiveness.
I'll second that. If you put air in the bottle it will begin to degrade the N and it will continue to degrade over time. The reason the lady in the video pushes air into the bottle is for the reason of exchanging the volume of air to the ratio of medicine she extracts and it is for pressurisation reasons like you suggested but with the 200ml bottles of N they are much thicker stronger glass and the small amount of liquid we take for testing is a very small percentage of the overall volume. Those small vials would be more prone to pressure issues. Chances are it won't brrak but eventually it would become harder to pull liquid from it with a syringe. If you were to intend to use the N frequently and take multiple doses that way from the bottle then yes, then you would probably need to start doing that exchange of air/volume ratio thing but in our cases, just for testing it's not necessary. Many have reported doing so without issue.
This is not correct procedure for testing. Do not inject air into the vial. I give myself injections for a medical condition and first thing I do is force the air out of the syringe. What she is demonstrating is not what you would be doing if you decide to test, which frankly I would recommend against.
You have told me about OCD or something that is causing you to continually question what you are being told. We are telling you that forcing air into your bottle will facilitate degradation of its effectiveness.
I'll second that. If you put air in the bottle it will begin to degrade the N and it will continue to degrade over time. The reason the lady in the video pushes air into the bottle is for the reason of exchanging the volume of air to the ratio of medicine she extracts and it is for pressurisation reasons like you suggested but with the 200ml bottles of N they are much thicker stronger glass and the small amount of liquid we take for testing is a very small percentage of the overall volume. Those small vials would be more prone to pressure issues. Chances are it won't brrak but eventually it would become harder to pull liquid from it with a syringe. If you were to intend to use the N frequently and take multiple doses that way from the bottle then yes, then you would probably need to start doing that exchange of air/volume ratio thing but in our cases, just for testing it's not necessary. Many have reported doing so without issue.
This is not correct procedure for testing. Do not inject air into the vial. I give myself injections for a medical condition and first thing I do is force the air out of the syringe. What she is demonstrating is not what you would be doing if you decide to test, which frankly I would recommend against.
You have told me about OCD or something that is causing you to continually question what you are being told. We are telling you that forcing air into your bottle will facilitate degradation of its effectiveness.
I think they just used a blunt needle because they weren't directly injecting with the needle used to draw it out. You can use a sharp needle. It's probably best to use a sharp one. There's no need to put it in at an angle. Just put it directly in. It's not complicated. You're really overthinking it. I assure you. You can use the method she uses in that video if you want to for peace of mind (going in at 45 degrees and using her technique) but just don't push air in. The reasons she uses that technique are explained. We're not using it intravenously therefore much of her precautions are not applicable for our needs.
I think they just used a blunt needle because they weren't directly injecting with the needle used to draw it out. You can use a sharp needle. It's probably best to use a sharp one. There's no need to put it in at an angle. Just put it directly in. It's not complicated. You're really overthinking it. I assure you. You can use the method she uses in that video if you want to for peace of mind (going in at 45 degrees and using her technique) but just don't push air in. The reasons she uses that technique are explained. We're not using it intravenously therefore much of her precautions are not applicable for our needs.
This site uses cookies to help personalise content, tailor your experience and to keep you logged in if you register.
By continuing to use this site, you are consenting to our use of cookies.